The seed of wild rice will automatically fall off when it matures, which is conducive to the spread and survival of the seeds. However, the "easy shattering property" has negative effects on the yield of harvested rice, for example, for cultivated rice varieties that target mature rice harvesting. Our ancestors began the process of domesticating rice early on from the point of view of the reduction in the number of falling grains. In fact, the seeds of modern cultivated rice differ significantly in their grain size, suggesting that shattering is a complex trait controlled by multiple genes. It is of great agricultural significance to understand in depth the comprehensive regulation mechanism of rice falling grains and reduce the yield loss caused by falling grains.

Researchers Han Bin and Zhou Yan, Lu Danfeng, and other researchers of the Institute of Phytobiology and National Genetics of the Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, introduced the fourth chromosome of wild rice W1943 into the background of cultivated rice, Guangluai No. 4. A material SL4 containing known shattering genes SH4 and qSH1 and exhibiting an easy falling-out was constructed. They conducted a -ray mutagenesis of SL4 to screen two mutants shhat1 and shat2 that were not completely shattered. Neither of these two mutants forms an aliquot, so the seeds need a lot of tension after they mature to separate the seeds from the branchlets. Through map-based cloning and genetic transformation validation, the researchers determined that the SHAT1 gene is an AP2 transcription factor that is highly homologous to the APETALA2 gene in Arabidopsis thaliana and is highly expressed in the stratified layer. Shat2 was identified as a new allelic mutation in the SH4 gene. Unlike the previously reported single amino acid substitution mutation type of sh4 in rice, shat2 is a frameshift mutation and is therefore named sh4-2.

The researchers further elaborated the genetic relationship between the three genes: SHAT1, SH4 and qSH1, through elaborate in situ hybridization analysis. SH4 promotes the expression of SHAT1 in stratified layers. In turn, SHA1 also plays a role in maintaining SH4 in stratified layers. The role of expression, both in the continuous expression of the separation from the layer is necessary for the correct formation of the layer. qSH1 acts downstream of SH4 and SHAT1 and promotes the formation of stratification by maintaining the constant expression of SHAT1 and SH4 in the stratified layer.

The study used a clever search for suppressor mutants to discover new rice down-regulation genes, and at the same time, it was linked to a known shrsp-regulated gene, opening up new research on rice shredding. Vision.

On March 9, the research paper "Genetic Control of Seed Shattering in Rice by the AP2 Transcription Factor SHATTERING ABORTION1" was published online in the international academic journal The Plant Cell. An editorial review article SHAT1, A new player in seed shattering of rice, was highly praised for this work.

Cutter Knife

Cutter Knife,Carpet Cutter Blades,Clever Cutter Knife,Sharp Cutter Knife

Yucheng County Ruiertuo Tools Co., Ltd. , https://www.ruiertuotools.com