Recently, the research group of the Institute of Intelligent Machinery, Institute of Hefei Institute of Physics, Chinese Academy of Sciences, Wang Suhua's team has made new progress in the detection of sulfur dioxide and bisulfite. This work establishes a rapid, sensitive and reversible method for the highly selective identification of sulfur dioxide and bisulfite, and designs and synthesizes a bisulfite-ratio reversible fluorescent probe. The probe can be used not only for the detection of sulfur dioxide in the atmosphere, but also for the imaging of intracellular bisulphite. Related research results have been published in the Journal of the American Chemical Society (Analytical Chemistry, 2016, 88, 4426−4431).

Excessive amounts of sulfur dioxide and bisulfite in the environment not only cause environmental hazards such as acid rain, but also seriously affect the human respiratory system and health. In the food industry, sulfur dioxide and bisulfite are also widely used as food bleaching agents and food preservatives, but excessive intake poses a serious hazard to human health. In vivo, it is one of the oxidative metabolites of biomolecules such as sulfur-containing amino acids. Pathological studies have found that the content of sulfite in patients with pneumonia and chronic renal failure increases significantly. Therefore, the development of highly sensitive and highly specific detection methods for sulfur dioxide and bisulfite is of great significance for environmental monitoring, food safety and related disease research.

In the aqueous system, the designed probes can react with sulfur dioxide or bisulfite rapidly and selectively, and cause the sensitive fluorescence of the probe system to change from rose to blue. Under UV light, the change in fluorescent color can be identified only with the naked eye. Hydrogen peroxide and other active oxygen can make the nucleophilic addition reaction of the system reversible change, red fluorescence recovery, and maintain good reactivity and recognition ability. The application of the reversible probe to the analysis of intracellular endogenous bisulphite was further explored. The results of the fluorescence imaging signal change indicated that the probe could recognize the reversible redox process and could be used in the intracellular oxidative stress process. Imaging research has a good application prospect for analyzing the pathogenic mechanism of oxidative stress process.

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